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gapdh (OriGene)

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OriGene gapdh
$SWE 160.1 treatment increased the antioxidant response. ( a ) Western blotting analysis of antioxidant factors (HO-1, SOD-2 <t>and</t> <t>Nrf2)</t> after 48 h of treatment with 75 and 100 µg GAE/mL SWE 160.1 in HBE cells. Protein levels were normalized to γ-tubulin. ( b ) qRT-PCR analysis of Nrf2, Keap1 and HO-1 mRNA levels after 24 h of treatment with 75 and 100 µg GAE/mL SWE 160.1 in HBE cells. Gene expression levels represent the relative mRNA expression compared to the untreated cells, normalized to <t>GAPDH</t> mRNA. ( c ) Western blotting of subcellular fractions of control and 48 h SWE 160.1 -treated cells incubated with pNrf2 (Ser40) antibody. Lamin A and GADPH antibodies marked as nuclei (N) and cytoplasmic (C) fractions, respectively. The images are representative of three different experiments. * p < 0.05, ** p < 0.01 vs. untreated control cells.$
Gapdh, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+gapdh/Nrf2+(NFE2L2)+Human+qPCR+Primer+Pair/pmc13209369-269-4-13
Average 94 stars, based on 2 article reviews

gapdh - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "Subcritical Water Extract from Grape Pomace Protects Human Bronchial Epithelium Cells by Mitigating Oxidative Stress Through Nrf2 Pathway"

Article Title: Subcritical Water Extract from Grape Pomace Protects Human Bronchial Epithelium Cells by Mitigating Oxidative Stress Through Nrf2 Pathway

Journal: Molecules

doi: 10.3390/molecules31101736

$SWE 160.1 treatment increased the antioxidant response. ( a ) Western blotting analysis of antioxidant factors (HO-1, SOD-2 and Nrf2) after 48 h of treatment with 75 and 100 µg GAE/mL SWE 160.1 in HBE cells. Protein levels were normalized to γ-tubulin. ( b ) qRT-PCR analysis of Nrf2, Keap1 and HO-1 mRNA levels after 24 h of treatment with 75 and 100 µg GAE/mL SWE 160.1 in HBE cells. Gene expression levels represent the relative mRNA expression compared to the untreated cells, normalized to GAPDH mRNA. ( c ) Western blotting of subcellular fractions of control and 48 h SWE 160.1 -treated cells incubated with pNrf2 (Ser40) antibody. Lamin A and GADPH antibodies marked as nuclei (N) and cytoplasmic (C) fractions, respectively. The images are representative of three different experiments. * p < 0.05, ** p < 0.01 vs. untreated control cells.$
Figure Legend Snippet: SWE 160.1 treatment increased the antioxidant response. ( a ) Western blotting analysis of antioxidant factors (HO-1, SOD-2 and Nrf2) after 48 h of treatment with 75 and 100 µg GAE/mL SWE 160.1 in HBE cells. Protein levels were normalized to γ-tubulin. ( b ) qRT-PCR analysis of Nrf2, Keap1 and HO-1 mRNA levels after 24 h of treatment with 75 and 100 µg GAE/mL SWE 160.1 in HBE cells. Gene expression levels represent the relative mRNA expression compared to the untreated cells, normalized to GAPDH mRNA. ( c ) Western blotting of subcellular fractions of control and 48 h SWE 160.1 -treated cells incubated with pNrf2 (Ser40) antibody. Lamin A and GADPH antibodies marked as nuclei (N) and cytoplasmic (C) fractions, respectively. The images are representative of three different experiments. * p < 0.05, ** p < 0.01 vs. untreated control cells.

Techniques Used: Western Blot, Quantitative RT-PCR, Gene Expression, Expressing, Control, Incubation

Mutagenesis:

Article Title: High throughput discovery of functional protein modifications by Hotspot Thermal Profiling.
Article Snippet: .. Full-length, human GAPDH (NM_002046, Origene) was used to generate GAPDH mutants using the QuikChange II Site-Directed Mutagenesis Kit according to the manufacturer’s protocol (catalog no. 200523, Agilent). ..

Article Title: High Throughput Discovery of Functional Protein Modifications by Hotspot Thermal Profiling
Article Snippet: .. Full-length, human GAPDH (NM_002046, Origene) was used to generate GAPDH mutants using the QuikChange II Site-Directed Mutagenesis Kit manufacturer protocol (200523, Agilent). ..

Sequencing:

Article Title: GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain.
Article Snippet: .. The cDNA sequence of human GAPDH (OriGene, UK) was inserted into the pET-28b(+) vector (Novagen). ..

Article Title: GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain
Article Snippet: .. The cDNA sequence of human GAPDH (OriGene, UK) was inserted into the pET-28b(+) vector (Novagen). ..

Article Title: GAPDH controls extracellular vesicle biogenesis and enhances therapeutic potential of EVs in silencing the Huntingtin gene in mice via siRNA delivery
Article Snippet: .. The cDNA sequence of human GAPDH (OriGene, UK) was inserted into the pET-28b(+) vector (Novagen). ..

Plasmid Preparation:

Western Blot:

Article Title: Myostatin Increases the Expression of Matrix Metalloproteinase Genes to Promote Preadipocytes Differentiation in Pigs
Article Snippet: 9 1Key Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei 10 Province, Institute of Animal Science and Veterinary Medicine, Hubei Academy of 11 Agricultural Sciences, Wuhan 430064, China 12 2Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan 13 3College of Life Science, South-Central University for Nationalities, Wuhan 430074, 14 China 15 4AgResearch Ltd., Ruakura Research Centre, Private Bag 3123, Hamilton 3214, New 16 Zealand 17 5 The University of Agriculture Dera Ismail Khan, Dera Ismail Khan, Pakistan 18 6These authors contributed equally to this work 19 *Corresponding: sukerbyz@126.com